ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
34.99 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Antigen NY-CO-7, CHIP, CLL-associated antigen KW-8, STUB1, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1, E3 ubiquitin-protein ligase CHIP, RING-type E3 ubiquitin transferase CHIP
背景
E3 ubiquitin-protein ligase CHIP (STUB1) is a ~34 kDa protein. E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Plays a role in the maintenance of mitochondrial morphology and promotes mitophagic removal of dysfunctional mitochondria; thereby acts as a protector against apoptosis in response to cellular stress. Negatively regulates vascular smooth muscle contraction, via degradation of the transcriptional activator MYOCD and subsequent loss of transcription of genes involved in vascular smooth muscle contraction. Promotes survival and proliferation of cardiac smooth muscle cells via ubiquitination and degradation of FOXO1, resulting in subsequent repression of FOXO1-mediated transcription of pro-apoptotic genes. Ubiquitinates ICER-type isoforms of CREM and targets them for proteasomal degradation, thereby acts as a positive effector of MAPK/ERK-mediated inhibition of apoptosis in cardiomyocytes.
1. Ballinger, CA. et al. (1999) Molecular and cellular biology 19, 4535-45. PMID: 10330192
2. Connell, P. et al. (2001) Nature cell biology 3, 93-6. PMID: 11146632
3. Jiang, J. et al. (2001) The Journal of biological chemistry 276, 42938-44. PMID: 11557750
4. Matsumura, Y. et al. (2013) The Journal of biological chemistry 288, 31069-79. PMID: 23990462
5. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139
6. Li, F. et al. (2009) The Journal of biological chemistry 284, 20090-8. PMID: 19483080
7. Woo, CH. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 4917-28. PMID: 20724525
8. Chao, CN. et al. (2019) Journal of cellular physiology 234, 20128-20138. PMID: 30980393
10. Parsons, JL. et al. (2009) The EMBO journal 28, 3207-15. PMID: 19713937
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Human STUB1 Recombinant Protein (N-His)
