ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
15.17 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4
背景
DNA repair protein XRCC4 is a ~38 kDa protein. DNA non-homologous end joining (NHEJ) core factor, required for double-strand break repair and V(D)J recombination. Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs). Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors.
1. Nick McElhinny, SA. et al. (2000) Molecular and cellular biology 20, 2996-3003. PMID: 10757784
2. Chen, L. et al. (2000) The Journal of biological chemistry 275, 26196-205. PMID: 10854421
3. Lee, JW. et al. (2003) Cancer research 63, 22-4. PMID: 12517771
4. Foster, RE. et al. (2006) Biochemical and biophysical research communications 341, 175-83. PMID: 16412978
5. Mari, PO. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 18597-602. PMID: 17124166
6. Gu, J. et al. (2007) The EMBO journal 26, 1010-23. PMID: 17290226
7. Roy, S. et al. (2012) Nucleic acids research 40, 1684-94. PMID: 22228831
8. Wanotayan, R. et al. (2015) Biochemical and biophysical research communications 457, 526-31. PMID: 25597996
9. de Bruin, C. et al. (2015) The Journal of clinical endocrinology and metabolism 100, E789-98. PMID: 25742519
10. Fukuchi, M. et al. (2015) Biochemical and biophysical research communications 461, 687-94. PMID: 25934149
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human XRCC4 Protein, N-His
