ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
23.66 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
S31I125, Tmp-21-I, Transmembrane protein Tmp21, Transmembrane emp24 domain-containing protein 10, TMP21, TMED10, 21 kDa transmembrane-trafficking protein, p23, S31III125, Protein TMED10, p24delta1, p24 family protein delta-1, p24delta
背景
Transmembrane emp24 domain-containing protein 10 (TMED10) is a ~24 kDa protein. Cargo receptor involved in protein vesicular trafficking and quality control in the endoplasmic reticulum (ER) and Golgi. The p24 protein family is a group of transmembrane proteins that bind coat protein complex I/COPI and coat protein complex II/COPII involved in vesicular trafficking between the membranes. Acts at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and involved in vesicle coat formation at the cytoplasmic side. Mainly functions in the early secretory pathway and cycles between the ER, ER-Golgi intermediate compartment (ERGIC) and Golgi, mediating cargo transport through COPI and COPII-coated vesicles. In COPII vesicle-mediated anterograde transport, involved in the transport of GPI-anchored proteins by acting together with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER.
1. Bremser, M. et al. (1999) Cell 96, 495-506. PMID: 10052452
2. Gommel, DU. et al. (2001) The EMBO journal 20, 6751-60. PMID: 11726511
3. Chen, F. et al. (2006) Nature 440, 1208-12. PMID: 16641999
4. Vetrivel, KS. et al. (2007) Molecular neurodegeneration 2, 4. PMID: 17288597
5. Luo, R. et al. (2009) Cellular signalling 21, 1169-79. PMID: 19296914
6. Bonnon, C. et al. (2010) Journal of cell science 123, 1705-15. PMID: 20427317
7. Luo, W. et al. (2011) Journal of neurochemistry 117, 71-81. PMID: 21219331
8. Nagae, M. et al. (2016) Journal of molecular biology 428, 4087-4099. PMID: 27569046
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human TMED10 Protein, N-His-SUMO
