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Bet v 1 Recombinant Protein (N-His) (PR095012)

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规格:
  • 50ug
  • 100ug
  • 1mg
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  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号PR095012
品牌abinScience
描述
Recombinant European white birch Bet v 1/Allergen Bet v I-A Protein, N-His (PR095012) expressed in E. coli, spanning Met1-Asn160. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
表达系统E. coli
Accession号P15494
蛋白长度Met1-Asn160
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Betula pendula (European white birch) (Betula verrucosa)
性质Recombinant
经测试应用WB
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
预测分子量19.87 kDa
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Allergen Bet v I-A, BETVI, BETVIA, Bet v 1-A, Major pollen allergen Bet v 1-A
背景

Major pollen allergen Bet v 1-A (Allergen Bet v I-A) is a ~17 kDa protein. May be a general steroid carrier protein.

NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Recombinant European white birch Bet v 1/Allergen Bet v I-A Protein, N-His

  • Bioactivity

    Detected by Anti-Bet v 1/Allergen Bet v I-A Antibody (SAA0656) (Cat No.: PR095023) in indirect ELISA.

  • Western blot

    Detected by Anti-Bet v 1 monoclonal antibody (Catalog No: PR095173)

  • Bioactivity

    Detected by Anti-Bet v 1/Allergen Bet v I-A Antibody (H3-1) (Catalog No: PR095173) in indirect ELISA

  • Bioactivity

    Detected by Bremzalerbart (Catalog No: PR095036) in indirect ELISA.

  • Bioactivity

    Detected by Atisnolerbart (Catalog No: PR095026) in indirect ELISA.

  • Bioactivity

    Detected by Umesolerbart (Catalog No: PR095016) in indirect ELISA.

  • Bioactivity

    Detected by SAA0666 (Catalog No: PR095123) in indirect ELISA.

  • Bioactivity

    Detected by SAA0667 (Catalog No: PR095133) in indirect ELISA.

  • Bioactivity

    Detected by SAA0668 (Catalog No: PR095143) in indirect ELISA.

  • Bioactivity

    Detected by SAA0668 (Catalog No: PR095183) in indirect ELISA.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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