ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
16.99 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Sigma-2 receptor, Sigma intracellular receptor 2, Meningioma-associated protein 30, MAC30, Transmembrane protein 97, S2R, TMEM97, Sigma2 receptor
背景
Sigma intracellular receptor 2 (TMEM97) is a ~20 kDa protein. Sigma-2 receptor which contributes to ameliorate dysfunctional cellular processes and slow degenerative progression by regulating cell functions including cholesterol biosynthesis/trafficking, membrane trafficking, autophagy, lipid membrane-bound protein trafficking, and receptor stabilization at the cell surface (Probable). Forms a ternary complex with PGRMC1 receptor and low density lipoprotein receptor/LDLR at the plasma membrane, which increases LDLR-mediated LDL cholesterol internalization. Decreases lysosomal sterol transporter NPC1 availability to the cell, probably through NPC1-binding, hence controlling lipid transport, including cholesterol and LBPA, outside of late endosome/lysosome. Binds regio- and stereoselective ligand 20(S)-hydroxycholesterol (20(S)-OHC) which enhances TMEM97-NPC1 interaction and decreases TMEM97-PGRMC1 and TMEM97-TSPO interactions, thereby linking OHC binding to cholesterol homeostasis. Also able to bind cholesterol.
1. Bartz, F. et al. (2009) Cell metabolism 10, 63-75. PMID: 19583955
2. Huang, YS. et al. (2014) Medicinal research reviews 34, 532-66. PMID: 23922215
3. Guo, L. et al. (2015) Current medicinal chemistry 22, 989-1003. PMID: 25620095
4. Ebrahimi-Fakhari, D. et al. (2016) Human molecular genetics 25, 3588-3599. PMID: 27378690
5. Alon, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 7160-7165. PMID: 28559337
6. Riad, A. et al. (2018) Scientific reports 8, 16845. PMID: 30443021
7. Son, KN. et al. (2021) The FEBS journal 288, 6815-6827. PMID: 34233061
8. Cheng, YS. et al. (2021) Nature chemical biology 17, 1271-1280. PMID: 34799735
9. Ma, D. et al. (2022) International journal of oral science 14, 42. PMID: 35970844
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human TMEM97 Protein, N-His-SUMO
