Caenorhabditis elegans HECD-1 Recombinant Protein (N-Strep) (JN857011)
概述
图片
参考文献
产品说明书
概述
货号
JN857011
品牌
abinScience
描述
Recombinant Caenorhabditis elegans HECD-1 Protein, N-Strep (JN857011) expressed in Insect Cells, spanning Met1-Asn2648. Purity: >90% by SDS-PAGE.
Highlights
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Insect cells
Accession号
V6CLA2
蛋白长度
Met1-Asn2648
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Caenorhabditis elegans
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
295.58 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
E3 ubiquitin-protein ligase hecd-1 is a ~292 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the ubiquitination and proteasomal-mediated degradation of cytoplasmic and mitochondrial proteins. Positively regulates lin-12 activity in the anchor cell (AC)/vulval precursor (VU) cell fate decision. Negatively regulates glp-1 activity in germline proliferation. May play a role in the formation of fibrous organelles, a hemidesmosome-like structure attaching muscles to the epidermis.
1. Liu, G. et al. (2011) The EMBO journal 30, 2990-3003. PMID: 21673654
2. Segref, A. et al. (2014) Cell metabolism 19, 642-52. PMID: 24703696
3. Chen, Y. et al. (2014) G3 (Bethesda, Md.) 5, 353-9. PMID: 25552605
4. Zahreddine, H. et al. (2010) Current biology : CB 20, 322-7. PMID: 20153198
5. Ackermann, L. et al. (2016) Nature structural & molecular biology 23, 995-1002. PMID: 27669035
Note
For research use only.
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SDS-PAGE
SDS-PAGE for Recombinant Caenorhabditis elegans HECD-1 Protein, N-Strep
