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Candida albicans CSA2 Recombinant Protein (C-His) (JN022011)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号JN022011
品牌abinScience
描述
Recombinant Candida albicans CSA2 Protein, C-His (JN022011) expressed in Yeast, spanning Asn34-Asn147. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • High Purity — >90% purity verified by SDS-PAGE.
表达系统Yeast cells
Accession号Q5A0X8
蛋白长度Asn34-Asn147
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Candida albicans
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
预测分子量22.49 kDa
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Secreted hemophore CSA2, Surface antigen protein 2, CSA2, CRW1, CAALFM_C406920CA, CaO19.10629, CaO19.3117
背景

Secreted hemophore CSA2 is a ~15 kDa protein. Secreted heme-binding protein involved in the utilization of iron from human hemoglobin during hyphal growth. May also play a role in non-hemoglobin iron utilization. Heme transfer occurs between PGA7, RBT5 and CSA2 supporting a model in which the 3 CFEM proteins cooperate in a heme-acquisition system and form a cross-cell wall heme-transfer cascade. The ability to acquire iron from host tissues is a major virulence factor of pathogenic microorganisms.

1. Okamoto-Shibayama, K. et al. (2014) FEMS yeast research 14, 674-7. PMID: 24796871
2. Nasser, L. et al. (2016) Nature microbiology 1, 16156. PMID: 27617569
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Recombinant Candida albicans CSA2 Protein, C-His

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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