ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
26.11 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Angiopoietin-related protein 3, Angiopoietin-like protein 3, ANGPTL3(17-224), Angptl3
背景
Angiopoietin-related protein 3 (ANGPTL3) is a ~52 kDa protein. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity; the function seems to be specific for the feeding conditions. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1. Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids; the cleaved N-terminal domain is more efficient than the uncleaved proprotein.
1. Koishi, R. et al. (2002) Nature genetics 30, 151-7. PMID: 11788823
2. Ando, Y. et al. (2003) Journal of lipid research 44, 1216-23. PMID: 12671033
3. Wang, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 11630-5. PMID: 26305978
4. Ono, M. et al. (2003) The Journal of biological chemistry 278, 41804-9. PMID: 12909640
5. Köster, A. et al. (2005) Endocrinology 146, 4943-50. PMID: 16081640
6. Liu, J. et al. (2010) The Journal of biological chemistry 285, 27561-70. PMID: 20581395
7. Jin, W. et al. (2007) Cell metabolism 6, 129-36. PMID: 17681148
8. Wang, Y. et al. (2015) Journal of lipid research 56, 1296-307. PMID: 25954050
9. Kim, HK. et al. (2015) Diabetes 64, 1142-53. PMID: 25338813
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Mouse ANGPTL3 Protein, N-His
