ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
25.26 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
ATP-dependent DNA helicase Q5, 5.6.2.4, DNA 3'-5' helicase RecQ5, DNA helicase, RecQ-like type 5, RecQ5, RecQ protein-like 5, RECQL5, RECQ5
背景
ATP-dependent DNA helicase Q5 (RECQL5) is a ~108 kDa protein. DNA helicase that plays an important role in DNA replication, transcription and repair. Probably unwinds DNA in a 3'-5' direction (Probable). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability. Also associates with POLR1A and enforces the stability of ribosomal DNA arrays. Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress.
1. Blundred, R. et al. (2010) DNA repair 9, 964-75. PMID: 20643585
2. Tadokoro, T. et al. (2012) Molecular biology of the cell 23, 4273-85. PMID: 22973052
3. Newman, JA. et al. (2017) Nucleic acids research 45, 4231-4243. PMID: 28100692
4. Islam, MN. et al. (2010) Molecular and cellular biology 30, 2460-72. PMID: 20231364
5. Urban, V. et al. (2016) The Journal of cell biology 214, 401-15. PMID: 27502483
6. Ramamoorthy, M. et al. (2012) Nucleic acids research 40, 1621-35. PMID: 22013166
7. Di Marco, S. et al. (2017) Molecular cell 66, 658-671.e8. PMID: 28575661
8. Ramamoorthy, M. et al. (2013) Carcinogenesis 34, 2218-30. PMID: 23715498
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human RECQL5 Protein, N-His
