Recombinant Human FUT3 Protein, C-His (HA398011) expressed in Mammalian Cells, spanning Arg35-Thr361.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P21217
蛋白长度
Arg35-Thr361
应用
SDS-PAGE, WB, ELISA, Immunogen, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>95% as determined by SDS-PAGE.
预测分子量
41.49 kDa
状态
Lyophilized
保存溶液
Lyophilized from PBS pH7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, EC:2.4.1.65, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, EC:2.4.1.152, Alpha-3-fucosyltransferase FUT3, 2.4.1.-, Blood group Lewis alpha-4-fucosyltransferase, Lewis FT, Fucosyltransferase 3, Fucosyltransferase III, FucT-III, FUT3
背景
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3 is a ~42 kDa protein. Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to both the subterminal N-acetyl glucosamine (GlcNAc) of type 1 chain (beta-D-Gal-(1->3)-beta-D-GlcNAc) glycolipids and oligosaccharides via an alpha(1,4) linkage, and the subterminal glucose (Glc) or GlcNAc of type 2 chain (beta-D-Gal-(1->4)-beta-D-GlcNAc) oligosaccharides via an alpha(1,3) linkage, independently of the presence of terminal alpha-L-fucosyl-(1,2) moieties on the terminal galactose of these acceptors. Through its catalytic activity, participates in the synthesis of antigens of the Lewis blood group system, i.e. Lewis a (Le(a)), lewis b (Le(b)), Lewis x/SSEA-1 (Le(x)) and lewis y (Le(y)) antigens. Also catalyzes the transfer of L-fucose to subterminal GlcNAc of sialyl- and disialyl-lactotetraosylceramide to produce sialyl Lewis a (sLe(a)) and disialyl Lewis a via an alpha(1,4) linkage and therefore may regulate cell surface sLe(a) expression and consequently regulates adhesive properties to E-selectin, cell proliferation and migration. Catalyzes the transfer of an L-fucose to 3'-sialyl-N-acetyllactosamine by an alpha(1,3) linkage, which allows the formation of sialyl-Lewis x structure and therefore may regulate the sialyl-Lewis x surface antigen expression and consequently adhesive properties to E-selectin.
1. Aubert, M. et al. (2000) International journal of cancer 88, 558-65. PMID: 11058871
2. Tsuchida, A. et al. (2003) The Journal of biological chemistry 278, 22787-94. PMID: 12668675
3. Kukowska-Latallo, JF. et al. (1990) Genes & development 4, 1288-303. PMID: 1977660
4. Cai, YJ. et al. (2016) Cellular and molecular biology (Noisy-le-Grand, France) 62, 15-20. PMID: 27453266
5. Mondal, N. et al. (2018) The Journal of biological chemistry 293, 7300-7314. PMID: 29593094
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human FUT3 Protein, C-His
