Recombinant Human LAMTOR5 Protein, N-His (PX-P5814)
概述
图片
参考文献
概述
货号
PX-P5814
品牌
ProteoGenix
描述
Recombinant Human LAMTOR5 Protein, N-His (PX-P5814) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
O43504
蛋白长度
Met1-Ser91
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
12.38 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
HBX-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5, HBXIP, LAMTOR5, HBV X-interacting protein, XIP, Ragulator complex protein LAMTOR5, Hepatitis B virus X-interacting protein
背景
Ragulator complex protein LAMTOR5 (HBXIP) is a ~9 kDa protein. As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. When complexed to BIRC5, interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby selectively suppressing apoptosis initiated via the mitochondrial/cytochrome c pathway.
1. Bar-Peled, L. et al. (2012) Cell 150, 1196-208. PMID: 22980980
2. Yonehara, R. et al. (2017) Nature communications 8, 1625. PMID: 29158492
3. Shen, K. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 9545-9550. PMID: 30181260
4. de Araujo, MEG. et al. (2017) Science (New York, N.Y.) 358, 377-381. PMID: 28935770
5. Su, MY. et al. (2017) Molecular cell 68, 835-846.e3. PMID: 29107538
