Recombinant Human PRKN Protein, N-His (PX-P5895)

概述
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参考文献

概述

货号	PX-P5895
品牌	ProteoGenix
描述	Recombinant Human PRKN Protein, N-His (PX-P5895) expressed in E. coli, Purity: >90% as determined by SDS-PAGE.. Highlights High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy. E. coli Expression — Optimized for functional studies.
表达系统	E. coli
Accession号	O60260
蛋白长度	Ile229-Val465
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Homo sapiens (Human)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	29.01 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	Parkin, PARK2, Parkinson juvenile disease protein 2, E3 ubiquitin-protein ligase parkin, Parkin RBR E3 ubiquitin-protein ligase, PRKN, Parkinson disease protein 2
背景	E3 ubiquitin-protein ligase parkin (PRKN) is a ~51 kDa protein. Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Substrates include SYT11 and VDAC1. Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2. Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. 1. Shimura, H. et al. (2000) Nature genetics 25, 302-5. PMID: 10888878 2. Imai, Y. et al. (2000) The Journal of biological chemistry 275, 35661-4. PMID: 10973942 3. Shimura, H. et al. (2001) Science (New York, N.Y.) 293, 263-9. PMID: 11431533 4. Imai, Y. et al. (2002) Molecular cell 10, 55-67. PMID: 12150907 5. Staropoli, JF. et al. (2003) Neuron 37, 735-49. PMID: 12628165 6. Chung, KK. et al. (2004) Science (New York, N.Y.) 304, 1328-31. PMID: 15105460 7. Ko, HS. et al. (2005) The Journal of neuroscience : the official journal of the Society for Neuroscience 25, 7968-78. PMID: 16135753 8. Shin, JH. et al. (2011) Cell 144, 689-702. PMID: 21376232 9. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592 10. Liu, S. et al. (2012) PLoS genetics 8, e1002537. PMID: 22396657
Note	For research use only.

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参考文献

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Recombinant Human PRKN Protein, N-His (PX-P5895)

概述

图片

参考文献

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重组蛋白

抗体

试剂盒

实验相关试剂

Recombinant Human PRKN Protein, N-His (PX-P5895)

概述

图片

参考文献

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