Recombinant Human PIP4K2B/PIP4KII-beta Protein, N-His (PX-P6121)
概述
图片
参考文献
概述
货号
PX-P6121
品牌
ProteoGenix
描述
Recombinant Human PIP4K2B/PIP4KII-beta Protein, N-His (PX-P6121) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
P78356
蛋白长度
Ser2-Thr416
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
48.49 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, PtdIns(5)P-4-kinase isoform 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PIP4KII-beta, Diphosphoinositide kinase 2-beta, PI(5)P 4-kinase type II beta, PIP5K2B, PIP4K2B
背景
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (PIP4K2B/PIP4KII-beta) is a ~47 kDa protein. Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration. Its GTP-sensing activity is critical for metabolic adaptation. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3.
1. Sumita, K. et al. (2016) Molecular cell 61, 187-98. PMID: 26774281
2. Castellino, AM. et al. (1997) The Journal of biological chemistry 272, 5861-70. PMID: 9038203
