Recombinant Human RUVBL1 Protein, N-His (PX-P5908)
概述
图片
参考文献
概述
货号
PX-P5908
品牌
ProteoGenix
描述
Recombinant Human RUVBL1 Protein, N-His (PX-P5908) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
Q9Y265
蛋白长度
Met1-Lys456
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
52.54 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
RuvB-like 1 (RUVBL1) is a ~50 kDa protein. Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400.
1. Puri, T. et al. (2007) Journal of molecular biology 366, 179-92. PMID: 17157868
2. López-Perrote, A. et al. (2020) eLife 9. PMID: 33205750
3. Doyon, Y. et al. (2004) Molecular and cellular biology 24, 1884-96. PMID: 14966270
4. Obri, A. et al. (2014) Nature 505, 648-53. PMID: 24463511
5. Jin, J. et al. (2005) The Journal of biological chemistry 280, 41207-12. PMID: 16230350
6. Chen, L. et al. (2011) The Journal of biological chemistry 286, 11283-9. PMID: 21303910
