Recombinant Human EZH2 Protein, N-His (PX-P5711)

概述
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参考文献

概述

货号	PX-P5711
品牌	ProteoGenix
描述	Recombinant Human EZH2 Protein, N-His (PX-P5711) expressed in E. coli, Purity: >90% as determined by SDS-PAGE.. Highlights High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy. E. coli Expression — Optimized for functional studies.
表达系统	E. coli
Accession号	Q15910
蛋白长度	Arg608-Pro746
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Homo sapiens (Human)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	18.44 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	ENX-1, Enhancer of zeste homolog 2, EZH2, Lysine N-methyltransferase 6, KMT6, Histone-lysine N-methyltransferase EZH2
背景	Histone-lysine N-methyltransferase EZH2 is a ~85 kDa protein. Catalytic subunit of the PRC2/EED-EZH2 complex, a Polycomb group (PcG) complex that methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. 1. Bracken, AP. et al. (2003) The EMBO journal 22, 5323-35. PMID: 14532106 2. Cao, R. et al. (2004) Molecular cell 15, 57-67. PMID: 15225548 3. Pasini, D. et al. (2004) The EMBO journal 23, 4061-71. PMID: 15385962 4. Bracken, AP. et al. (2006) Genes & development 20, 1123-36. PMID: 16618801 5. Kim, DH. et al. (2006) Nature structural & molecular biology 13, 793-7. PMID: 16936726 6. Bracken, AP. et al. (2007) Genes & development 21, 525-30. PMID: 17344414 7. McCabe, MT. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 2989-94. PMID: 22323599 8. Chu, CS. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 1355-60. PMID: 24474760 9. Kim, JM. et al. (2015) Scientific reports 5, 16714. PMID: 26581166 10. Li, D. et al. (2018) Nature communications 9, 2829. PMID: 30026490
Note	For research use only.

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参考文献

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Recombinant Human EZH2 Protein, N-His (PX-P5711)

概述

图片

参考文献

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重组蛋白

抗体

试剂盒

实验相关试剂

Recombinant Human EZH2 Protein, N-His (PX-P5711)

概述

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参考文献

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