Recombinant Human MDA5/IFIH1 Protein, N-Strep (PX-P6326)
概述
图片
参考文献
概述
货号
PX-P6326
品牌
ProteoGenix
描述
Recombinant Human MDA5/IFIH1 Protein, N-Strep (PX-P6326) expressed in Insect Cells, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
Insect Cells Expression — Optimized for functional studies.
表达系统
Insect Cells
Accession号
Q9BYX4
蛋白长度
Asn191-Asp1025
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
96.80 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Interferon-induced helicase C domain-containing protein 1, 3.6.4.13, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2, RNA helicase-DEAD box protein 116, IFIH1, MDA5, RH116
背景
Interferon-induced helicase C domain-containing protein 1 (MDA5/IFIH1) is a ~116 kDa protein. Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus. Detects coronavirus SARS-CoV-2.
1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402
2. Wu, XM. et al. (2020) Journal of immunology (Baltimore, Md. : 1950) 204, 2216-2231. PMID: 32169843
3. Liu, G. et al. (2021) Nature microbiology 6, 467-478. PMID: 33727702
4. Peisley, A. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 21010-5. PMID: 22160685
5. Lamborn, IT. et al. (2017) The Journal of experimental medicine 214, 1949-1972. PMID: 28606988
