Recombinant Human TENT4B/GLD4 Protein, N-His (PX-P4250)
概述
图片
参考文献
概述
货号
PX-P4250
品牌
ProteoGenix
描述
Recombinant Human TENT4B/GLD4 Protein, N-His (PX-P4250) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
Q8NDF8
蛋白长度
Gly116-Gly433
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
38.69 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Terminal nucleotidyltransferase 4B (TENT4B/GLD4) is a ~63 kDa protein. Terminal nucleotidyltransferase that catalyzes preferentially the transfer of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1.
1. Rammelt, C. et al. (2011) RNA (New York, N.Y.) 17, 1737-46. PMID: 21788334
2. Lim, J. et al. (2018) Science (New York, N.Y.) 361, 701-704. PMID: 30026317
3. Lubas, M. et al. (2011) Molecular cell 43, 624-37. PMID: 21855801
4. Shin, J. et al. (2017) Nucleic acids research 45, 6793-6804. PMID: 28383716
5. Mullen, TE. et al. (2008) Genes & development 22, 50-65. PMID: 18172165
6. Boele, J. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 11467-72. PMID: 25049417
7. Berndt, H. et al. (2012) RNA (New York, N.Y.) 18, 958-72. PMID: 22442037
