Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
ESAT-6-like protein EsxB, 10 kDa culture filtrate antigen CFP-10, CFP-10, Secreted antigenic protein MTSA-10, esxB, cfp10, lhp, mtsA10, Rv3874, MTV027.09
背景
ESAT-6-like protein EsxB (CFP10) is a ~10 kDa protein. A secreted protein. Acts as a strong host (human) T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA; native protein binds poorly to artificial liposomes in the absence or presence of EsxA. EsxA and EsxA-EsxB are cytotoxic to pneumocytes.
1. Renshaw, PS. et al. (2002) The Journal of biological chemistry 277, 21598-603. PMID: 11940590
2. van der Wel, N. et al. (2007) Cell 129, 1287-98. PMID: 17604718
3. de Jonge, MI. et al. (2007) Journal of bacteriology 189, 6028-34. PMID: 17557817
4. Refai, A. et al. (2015) The FEBS journal 282, 4114-29. PMID: 26260636
