Recombinant Human LpPLA2/PLA2G7/PAF-AH Protein, C-His (PX-P6459)
概述
图片
参考文献
概述
货号
PX-P6459
品牌
ProteoGenix
描述
Recombinant Human LpPLA2/PLA2G7/PAF-AH Protein, C-His (PX-P6459) expressed in Mammalian Cells, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
Mammalian Cells Expression — Optimized for functional studies.
表达系统
Mammalian Cells
Accession号
Q13093
蛋白长度
Met1-Asn441 (Val379Ala)
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
51.02 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Platelet-activating factor acetylhydrolase (Lp-PLA2/PLA2G7) is a ~50 kDa protein. Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells.
1. Stafforini, DM. et al. (1999) The Journal of biological chemistry 274, 7018-24. PMID: 10066756
2. Stafforini, DM. et al. (2006) The Journal of biological chemistry 281, 4616-23. PMID: 16371369
3. Kriska, T. et al. (2007) The Journal of biological chemistry 282, 100-8. PMID: 17090529
4. Stremler, KE. et al. (1991) The Journal of biological chemistry 266, 11095-103. PMID: 2040620
