ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
33.94 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
ATP-binding cassette sub-family D member 1; 3.1.2.-; 7.6.2.-; Adrenoleukodystrophy protein; ALDP; ABCD1; ALD
背景
ATP-binding cassette sub-family D member 1 is a ~82 kDa protein. ATP-dependent transporter of the ATP-binding cassette (ABC) family involved in the transport of very long chain fatty acid (VLCFA)-CoA from the cytosol to the peroxisome lumen. Coupled to the ATP-dependent transporter activity also has a fatty acyl-CoA thioesterase activity (ACOT) and hydrolyzes VLCFA-CoA into VLCFA prior their ATP-dependent transport into peroxisomes, the ACOT activity is essential during this transport process. Thus, plays a role in regulation of VLCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation, mitochondrial function and microsomal fatty acid elongation. Involved in several processes; namely, controls the active myelination phase by negatively regulating the microsomal fatty acid elongation activity and may also play a role in axon and myelin maintenance. Also controls the cellular response to oxidative stress by regulating mitochondrial functions such as mitochondrial oxidative phosphorylation and depolarization.
1. Roerig, P. et al. (2001) FEBS letters 492, 66-72. PMID: 11248239
2. Guimarães, CP. et al. (2005) Journal of human genetics 50, 99-105. PMID: 15682271
3. Morita, M. et al. (2006) Biological & pharmaceutical bulletin 29, 1836-42. PMID: 16946495
4. van Roermund, CW. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 4201-8. PMID: 18757502
5. van Roermund, CW. et al. (2011) Biochimica et biophysica acta 1811, 148-52. PMID: 21145416
6. Wiesinger, C. et al. (2013) The Journal of biological chemistry 288, 19269-79. PMID: 23671276
7. Okamoto, T. et al. (2018) Biochemical and biophysical research communications 496, 1122-1127. PMID: 29397936
8. Kawaguchi, K. et al. (2021) Scientific reports 11, 2192. PMID: 33500543
Note
For research use only
图片
SDS-PAGE
SDS-PAGE for Recombinant Human ABCD1 Protein, N-His
