ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
34.59 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nuclear factor of activated T-cells, cytoplasmic 4 is a ~95 kDa protein. Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4. Along with NFATC3, involved in embryonic heart development. Following JAK/STAT signaling activation and as part of a complex with NFATC3 and STAT3, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes. Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function.
1. Yang, T. et al. (2001) The Journal of biological chemistry 276, 39569-76. PMID: 11514544
2. Yang, TT. et al. (2002) Molecular and cellular biology 22, 3892-904. PMID: 11997522
3. Cho, YY. et al. (2007) The Journal of biological chemistry 282, 8380-92. PMID: 17213202
4. Yao, K. et al. (2007) Cancer research 67, 8725-35. PMID: 17875713
5. Qin, X. et al. (2008) Cellular and molecular life sciences : CMLS 65, 2752-62. PMID: 18668201
6. Mei, Z. et al. (2015) Neurochemical research 40, 829-36. PMID: 25663301
7. Hoey, T. et al. (1995) Immunity 2, 461-72. PMID: 7749981
8. Yang, TT. et al. (2008) Molecular and cellular biology 28, 3489-501. PMID: 18347059
Note
For research use only
图片
SDS-PAGE
SDS-PAGE for Recombinant Human NFATC4 Protein, N-His
