ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
20.71 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
BRISC complex subunit Abraxas 2; Abraxas brother protein 1; Protein FAM175B; ABRAXAS2; ABRO1; FAM175B; KIAA0157
背景
BRISC complex subunit Abraxas 2 is a ~46 kDa protein. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked polyubiquitin, leaving the last ubiquitin chain attached to its substrates. May act as a central scaffold protein that assembles the various components of the BRISC complex and retains them in the cytoplasm. Plays a role in regulating the onset of apoptosis via its role in modulating 'Lys-63'-linked ubiquitination of target proteins. Required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activities by enhancing its stability and cell surface expression.
1. Cooper, EM. et al. (2009) The EMBO journal 28, 621-31. PMID: 19214193
2. Cooper, EM. et al. (2010) The Journal of biological chemistry 285, 10344-52. PMID: 20032457
3. Feng, L. et al. (2010) The Journal of biological chemistry 285, 30982-8. PMID: 20656690
4. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985
5. Yan, K. et al. (2015) The Journal of cell biology 210, 209-24. PMID: 26195665
6. Zeqiraj, E. et al. (2015) Molecular cell 59, 970-83. PMID: 26344097
7. Zhang, J. et al. (2014) Nature communications 5, 5059. PMID: 25283148
Note
For research use only
图片
SDS-PAGE
SDS-PAGE for Recombinant Human ABRAXAS2 Protein, N-His
