ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
43.12 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Ubiquitin carboxyl-terminal hydrolase 12 is a ~42 kDa protein. Deubiquitinating enzyme that plays various roles in the regulation of the immune response and inflammation. During TCR engagement and activation, translocates into the cytoplasm and deubiquitinates its substrates LAT and TRAT1 and prevents their lysosome-dependent degradation to stabilize the TCR signaling complex at the plasma membrane. Plays an essential role in the selective LPS-induced macrophage response through the activation of NF-kappa-B pathway. In addition, promotes that antiviral immune response through targeting DNA sensor IFI16 to inhibit its proteasome-dependent degradation. Participates in the interferon signaling pathway and antiviral response independently of its deubiquitinase activity by maintaining nuclear phosphorylated STAT1 levels via inhibition of its CREBBP-mediated acetylation and subsequent dephosphorylation.
1. Cohn, MA. et al. (2009) The Journal of biological chemistry 284, 5343-51. PMID: 19075014
2. Li, H. et al. (2016) Molecular cell 63, 249-260. PMID: 27373336
3. Jahan, AS. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, E705-14. PMID: 26811477
4. Nayak, TKS. et al. (2017) Biochemical and biophysical research communications 483, 69-74. PMID: 28063927
5. Fu, Y. et al. (2023) PLoS pathogens 19, e1011480. PMID: 37410794
6. Liu, J. et al. (2020) PLoS pathogens 16, e1008215. PMID: 31899788
7. Choi, HS. et al. (2022) International journal of molecular sciences 23. PMID: 36361894
8. Moretti, J. et al. (2012) The Journal of biological chemistry 287, 29429-41. PMID: 22778262
9. Aron, R. et al. (2018) Nature communications 9, 3191. PMID: 30266909
10. Gangula, NR. et al. (2013) The Journal of biological chemistry 288, 34545-54. PMID: 24145035
Note
For research use only
图片
SDS-PAGE
SDS-PAGE for Recombinant Human USP12 Protein, N-His
