ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
16.96 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
NELF-E, NELFE, Negative elongation factor E, RD, RDBP, RNA-binding protein RD
背景
Negative elongation factor E is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA.
1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401
2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882
3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650
4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062
