Human EIF5B Recombinant Protein (N-GST) (HC158012)

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100ug
1mg

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概述
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概述

货号	HC158012
品牌	abinScience
描述	Recombinant Human EIF5B Protein, N-GST (HC158012) expressed in E. coli, spanning Thr871-Ile1220. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	E. coli
Accession号	O60841
蛋白长度	Thr871-Ile1220
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Homo sapiens (Human)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	66.38 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	EC:3.6.5.3, EIF5B, Eukaryotic translation initiation factor 5B, IF2, KIAA0741, Translation initiation factor IF-2, eIF-5B
背景	Eukaryotic translation initiation factor 5B is a ~138 kDa protein. Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. 1. Pestova, TV. et al. (2000) Nature 403, 332-5. PMID: 10659855 2. Lapointe, CP. et al. (2022) Nature 607, 185-190. PMID: 35732735 3. Marintchev, A. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1535-40. PMID: 12569173
Note	For research use only

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参考文献

产品说明书

Human EIF5B Recombinant Protein (N-GST).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Human EIF5B Recombinant Protein (N-GST) (HC158012)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Human EIF5B Recombinant Protein (N-GST) (HC158012)

概述

图片

参考文献

产品说明书

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