Human LIN28B Recombinant Protein (N-His) (HC186012)

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概述

货号	HC186012
品牌	abinScience
描述	Recombinant Human LIN28B Protein, N-His (HC186012) expressed in E. coli, spanning Gln26-Gly101. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	E. coli
Accession号	Q6ZN17
蛋白长度	Gln26-Gly101
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Homo sapiens (Human)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	10.92 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	CSDD2, LIN28B, Lin-28B, Protein lin-28 homolog B
背景	Protein lin-28 homolog B is a ~27 kDa protein. Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. 1. Piskounova, E. et al. (2011) Cell 147, 1066-79. PMID: 22118463 2. Heo, I. et al. (2009) Cell 138, 696-708. PMID: 19703396
Note	For research use only

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参考文献

产品说明书

Human LIN28B Recombinant Protein (N-His).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Human LIN28B Recombinant Protein (N-His) (HC186012)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Human LIN28B Recombinant Protein (N-His) (HC186012)

概述

图片

参考文献

产品说明书

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