ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
26.31 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
OTRPC4, Osm-9-like TRP channel 4, TRP12, TRPV4, Transient receptor potential cation channel subfamily V member 4, Transient receptor potential protein 12, TrpV4, VR-OAC, VRL-2, VRL2, VROAC, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel
背景
Transient receptor potential cation channel subfamily V member 4 is a ~98 kDa protein. Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by heat, low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.
1. Arniges, M. et al. (2006) The Journal of biological chemistry 281, 1580-6. PMID: 16293632
2. Köttgen, M. et al. (2008) The Journal of cell biology 182, 437-47. PMID: 18695040
3. Garcia-Elias, A. et al. (2008) The Journal of biological chemistry 283, 31284-8. PMID: 18826956
4. Fiorillo, C. et al. (2012) Neurogenetics 13, 195-203. PMID: 22526352
5. Thorneloe, KS. et al. (2012) Science translational medicine 4, 159ra148. PMID: 23136043
6. Doñate-Macián, P. et al. (2018) Nature communications 9, 2307. PMID: 29899501
