ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
62.71 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
ACADVL, EC:1.3.8.9, VLCAD, Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
背景
Very long-chain acyl-CoA dehydrogenase, mitochondrial is a ~70 kDa protein. Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on fatty acyl-CoAs with saturated 12 to 24 carbons long primary chains.
1. He, M. et al. (2007) American journal of human genetics 81, 87-103. PMID: 17564966
2. McAndrew, RP. et al. (2008) The Journal of biological chemistry 283, 9435-43. PMID: 18227065
3. Aoyama, T. et al. (1995) American journal of human genetics 57, 273-83. PMID: 7668252
4. Souri, M. et al. (1998) The Journal of biological chemistry 273, 4227-31. PMID: 9461620
5. Souri, M. et al. (1998) FEBS letters 426, 187-90. PMID: 9599005
6. Souri, M. et al. (1998) European journal of biochemistry 257, 592-8. PMID: 9839948
7. He, M. et al. (2011) Molecular genetics and metabolism 102, 418-29. PMID: 21237683
