Recombinant Human CD69 Protein, C-Fc (HC273021) expressed in Mammalian Cells, spanning Ser62-Lys199. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
Q07108
蛋白长度
Ser62-Lys199
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
44.31 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, CD69, CLEC2C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leukocyte surface antigen Leu-23, MLR-3
背景
Early activation antigen CD69 is a ~22 kDa protein. Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Also acts via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells.
1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896
2. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369
3. Jiménez-Fernández, M. et al. (2022) Cellular and molecular life sciences : CMLS 79, 468. PMID: 35930205
4. Jiang, Y. et al. (2021) Clinical and translational medicine 11, e563. PMID: 34841721
