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Human DGKZ Recombinant Protein (N-His) (HC693032)

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  • 100ug
  • 1mg
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概述
货号HC693032
品牌abinScience
描述
Recombinant Human DGKZ Protein, N-His (HC693032) expressed in E. coli, spanning Gly411-Lys624. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
表达系统E. coli
Accession号Q13574
蛋白长度Gly411-Lys624
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Homo sapiens (Human)
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
预测分子量26.33 kDa
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名DAG kinase zeta, DAGK6, DGK-zeta, DGKZ, Diacylglycerol kinase zeta, Diglyceride kinase zeta, EC:2.7.1.107, EC:2.7.1.93
背景

Diacylglycerol kinase zeta is a ~103 kDa protein. Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group.

1. Epand, RM. et al. (2004) Biochemistry 43, 14778-83. PMID: 15544348
2. Epand, RM. et al. (2007) Biochemistry 46, 14225-31. PMID: 18004883
3. Lung, M. et al. (2009) The Journal of biological chemistry 284, 31062-73. PMID: 19744926
4. Shulga, YV. et al. (2011) FEBS letters 585, 4025-8. PMID: 22108654
5. Gellett, AM. et al. (2012) Biochemical and biophysical research communications 422, 758-63. PMID: 22627129
6. Sato, M. et al. (2013) Pharmacology 92, 99-107. PMID: 23949095
7. Ding, L. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 5519-24. PMID: 9159104
NoteFor research use only
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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