ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
36.68 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
KIAA0733, MAP3K7IP2, Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAB-2, TAB2, TAK1-binding protein 2, TGF-beta-activated kinase 1 and MAP3K7-binding protein 2, TGF-beta-activated kinase 1-binding protein 2
背景
TGF-beta-activated kinase 1 and MAP3K7-binding protein 2 is a ~76 kDa protein. Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains. The RanBP2-type zinc finger (NZF) specifically recognizes Lys-63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1 and RIPK2: this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1. Also recognizes and binds Lys-63'-linked polyubiquitin chains of heterotypic 'Lys-63'-/'Lys-48'-linked branched ubiquitin chains. Regulates the IL1-mediated translocation of NCOR1 out of the nucleus.
1. Takaesu, G. et al. (2000) Molecular cell 5, 649-58. PMID: 10882101
2. Wang, C. et al. (2001) Nature 412, 346-51. PMID: 11460167
3. Kanayama, A. et al. (2004) Molecular cell 15, 535-48. PMID: 15327770
4. Zhang, L. et al. (2011) Nature 481, 204-8. PMID: 22158122
5. Ohtake, F. et al. (2016) Molecular cell 64, 251-266. PMID: 27746020
6. Gu, Z. et al. (2021) Cellular & molecular immunology 18, 1981-1994. PMID: 33184450
7. Zhang, J. et al. (2023) Cell death and differentiation 30, 966-978. PMID: 36681779
