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Human TRMT112 Recombinant Protein (C-Strep) (HC763012)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
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概述
货号HC763012
品牌abinScience
描述
Recombinant Human TRMT112 Protein, C-Strep (HC763012) expressed in E. coli, spanning Met1-Ser125. Purity: >90% by SDS-PAGE.
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
表达系统E. coli
Accession号Q9UI30
蛋白长度Met1-Ser125
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Homo sapiens (Human)
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
预测分子量15.37 kDa
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Multifunctional methyltransferase subunit TRM112-like protein, TRMT112, tRNA methyltransferase 112 homolog
背景

Multifunctional methyltransferase subunit TRM112-like protein is a ~14 kDa protein. Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase BUD23, methylates the N(7) position of a guanine in 18S rRNA. The heterodimer with N6AMT1/HEMK2 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. The heterodimer with N6AMT1/HEMK2 also monomethylates 'Lys-12' of histone H4 (H4K12me1). The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species.

1. Figaro, S. et al. (2008) FEBS letters 582, 2352-6. PMID: 18539146
2. Fu, D. et al. (2010) Molecular and cellular biology 30, 2449-59. PMID: 20308323
3. Zorbas, C. et al. (2015) Molecular biology of the cell 26, 2080-95. PMID: 25851604
4. Metzger, E. et al. (2019) Nature structural & molecular biology 26, 361-371. PMID: 31061526
5. van Tran, N. et al. (2019) Nucleic acids research 47, 7719-7733. PMID: 31328227
6. Li, W. et al. (2019) Cell discovery 5, 51. PMID: 31636962
7. Wang, C. et al. (2023) Nucleic acids research 51, 7496-7519. PMID: 37283053
9. Yu, D. et al. (2021) The Journal of biological chemistry 296, 100270. PMID: 33428944
10. Sepich-Poore, C. et al. (2022) The Journal of biological chemistry 298, 101590. PMID: 35033535
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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