ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
39.16 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
E3 ubiquitin-protein ligase synoviolin is a ~67 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation.
1. Kaneko, M. et al. (2002) FEBS letters 532, 147-52. PMID: 12459480
2. Nadav, E. et al. (2003) Biochemical and biophysical research communications 303, 91-7. PMID: 12646171
3. Amano, T. et al. (2003) Genes & development 17, 2436-49. PMID: 12975321
4. Kikkert, M. et al. (2004) The Journal of biological chemistry 279, 3525-34. PMID: 14593114
5. Schulze, A. et al. (2005) Journal of molecular biology 354, 1021-7. PMID: 16289116
6. Arteaga, MF. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 11178-83. PMID: 16847254
7. Omura, T. et al. (2006) Journal of neurochemistry 99, 1456-69. PMID: 17059562
8. Yang, H. et al. (2007) Experimental cell research 313, 538-50. PMID: 17141218
9. Yamasaki, S. et al. (2007) The EMBO journal 26, 113-22. PMID: 17170702
10. Sato, T. et al. (2012) Molecular cell 47, 99-110. PMID: 22607976
