ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
22.96 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, EC:2.3.2.27, KIAA0714, LTN1, RING finger protein 160, RING-type E3 ubiquitin transferase listerin, RNF160, ZNF294, Zinc finger protein 294
背景
E3 ubiquitin-protein ligase listerin is a ~200 kDa protein. E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Within the RQC complex, LTN1 is recruited to stalled 60S ribosomal subunits by NEMF and mediates ubiquitination of stalled nascent chains. Ubiquitination leads to VCP/p97 recruitment for extraction and degradation of the incomplete translation product.
1. Shao, S. et al. (2013) Molecular cell 50, 637-48. PMID: 23685075
2. Shao, S. et al. (2014) Molecular cell 55, 880-890. PMID: 25132172
3. Shao, S. et al. (2015) Molecular cell 57, 433-44. PMID: 25578875
4. Matsuo, Y. et al. (2017) Nature communications 8, 159. PMID: 28757607
