ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
31.14 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
15-PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD(+)], EC:1.1.1.-, EC:1.1.1.141, EC:1.1.1.232, Eicosanoid/docosanoid dehydrogenase [NAD(+)], HPGD, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1, Short chain dehydrogenase/reductase family 36C member 1
背景
15-hydroxyprostaglandin dehydrogenase [NAD(+)] is a ~28 kDa protein. Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites. Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression. Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.
1. Clish, CB. et al. (2000) The Journal of biological chemistry 275, 25372-80. PMID: 10837478
2. Arita, M. et al. (2006) The Journal of biological chemistry 281, 22847-54. PMID: 16757471
3. Cho, H. et al. (2006) Bioorganic & medicinal chemistry 14, 6486-91. PMID: 16828555
4. Liu, X. et al. (2011) Chemical research in toxicology 24, 2227-36. PMID: 21916491
5. Wendell, SG. et al. (2015) The Journal of biological chemistry 290, 5868-80. PMID: 25586183
6. Ensor, CM. et al. (1994) Biochimica et biophysica acta 1208, 151-6. PMID: 8086429
7. Yan, M. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 17468-73. PMID: 15574495
8. Clària, J. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 2597-605. PMID: 22844113
