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Human ZDHHC1 Recombinant Protein (N-His-KSI) (HD005012)

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  • 50ug
  • 100ug
  • 1mg
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概述
货号HD005012
品牌abinScience
描述
Recombinant Human ZDHHC1 Protein, N-His-KSI (HD005012) expressed in E. coli, spanning His263-Ser485. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
表达系统E. coli
Accession号Q8WTX9
蛋白长度His263-Ser485
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Homo sapiens (Human)
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
预测分子量41.60 kDa
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名C16orf1, DHHC domain-containing cysteine-rich protein 1, DHHC-1, EC:2.3.1.225, Palmitoyltransferase ZDHHC1, ZDHHC1, ZNF377, Zinc finger DHHC domain-containing protein 1, Zinc finger protein 377
背景

Palmitoyltransferase ZDHHC1 is a ~54 kDa protein. Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates, such as NCDN and NLRP3. Has a palmitoyltransferase activity toward NCDN and regulates NCDN association with endosome membranes through this palmitoylation. Acts as an activator of the NLRP3 inflammasome by mediating palmitoylation of 'Cys-130' and 'Cys-958' of NLRP3, thereby promoting NLRP3 phosphorylation and activation by NEK7.

1. Nie, L. et al. (2024) Molecular cell 84, 3336-3353.e7. PMID: 39173637
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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