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Human UGT1A3 Recombinant Protein (N-His) (HD018012)

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  • 1mg
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概述
货号HD018012
品牌abinScience
描述
Recombinant Human UGT1A3 Protein, N-His (HD018012) expressed in E. coli, spanning Gly29-Asp491. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
表达系统E. coli
Accession号P35503
蛋白长度Gly29-Asp491
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Homo sapiens (Human)
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
预测分子量54.88 kDa
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名EC:2.4.1.17, GNT1, UDP-glucuronosyltransferase 1-3, UDP-glucuronosyltransferase 1-C, UDP-glucuronosyltransferase 1A isoform 3, UDP-glucuronosyltransferase 1A3, UDPGT 1-3, UGT-1C, UGT1, UGT1*3, UGT1-03, UGT1.3, UGT1A3, UGT1C
背景

UDP-glucuronosyltransferase 1A3 is a ~60 kDa protein. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol and estrone. Contributes to bile acid (BA) detoxification by catalyzing the glucuronidation of BA substrates, which are natural detergents for dietary lipids absorption. Involved in the glucuronidation of calcidiol, which is the major circulating form of vitamin D3, essential for the regulation of calcium and phosphate homeostasis.

1. Lépine, J. et al. (2004) The Journal of clinical endocrinology and metabolism 89, 5222-32. PMID: 15472229
2. Alonen, A. et al. (2008) Biochemical pharmacology 76, 763-72. PMID: 18674515
3. Itäaho, K. et al. (2008) Drug metabolism and disposition: the biological fate of chemicals 36, 2307-15. PMID: 18719240
4. Sneitz, N. et al. (2013) Drug metabolism and disposition: the biological fate of chemicals 41, 582-91. PMID: 23288867
5. Perreault, M. et al. (2013) Drug metabolism and disposition: the biological fate of chemicals 41, 1616-20. PMID: 23756265
6. Wang, Z. et al. (2014) Endocrinology 155, 2052-63. PMID: 24641623
7. Li, X. et al. (2011) Drug metabolism and pharmacokinetics 26, 341-50. PMID: 21422672
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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