ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
37.08 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
EC:2.5.1.60, GGTB, GGTase-II-beta, Geranylgeranyl transferase type II subunit beta, Geranylgeranyl transferase type-2 subunit beta, RABGGTB, Rab GG transferase beta, Rab GGTase beta, Rab geranyl-geranyltransferase subunit beta, Rab geranylgeranyltransferase subunit beta, Type II protein geranyl-geranyltransferase subunit beta
背景
Geranylgeranyl transferase type-2 subunit beta is a ~36 kDa protein. Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A. Catalytic subunit of the geranylgeranyl transferase type 3 (GGTase-3) complex. The GGTase-3 complex geranylgeranylates and targets FBXL2 to the cellular membranes, where FBXL2 forms part of the E3 ubiquitin-protein ligase complex SCF(FBXL2) that mediates the degradation of membrane-anchored proteins. The GGTase-3 complex geranylgeranylates Golgi v-SNARE protein YKT6 at 'Cys-194' and this prenylation is required for Golgi SNARE complex assembly.
1. Farnsworth, CC. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 11963-7. PMID: 7991565
2. Kuchay, S. et al. (2019) Nature structural & molecular biology 26, 628-636. PMID: 31209342
3. Shirakawa, R. et al. (2020) The EMBO journal 39, e104120. PMID: 32128853
