ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
20.10 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
AdPLA, Adipose-specific phospholipase A2, EC:2.3.1.-, EC:3.1.1.32, EC:3.1.1.4, Group XVI phospholipase A1/A2, H-REV107, H-rev 107 protein homolog, HRAS-like suppressor 1, HRAS-like suppressor 3, HRASLS3, HREV107, HREV107-1, HREV107-3, HRSL3, PLA2G16, PLAAT3, Phospholipase A and acyltransferase 3, Renal carcinoma antigen NY-REN-65
背景
Phospholipase A and acyltransferase 3 is a ~17 kDa protein. Exhibits both phospholipase A1/2 and acyltransferase activities. Shows phospholipase A1 (PLA1) and A2 (PLA2) activity, catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids. For most substrates, PLA1 activity is much higher than PLA2 activity. Shows O-acyltransferase activity,catalyzing the transfer of a fatty acyl group from glycerophospholipid to the hydroxyl group of lysophospholipid. Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs).
1. Uyama, T. et al. (2009) Journal of lipid research 50, 685-93. PMID: 19047760
2. Uyama, T. et al. (2009) Biochimica et biophysica acta 1791, 1114-24. PMID: 19615464
3. Golczak, M. et al. (2012) The Journal of biological chemistry 287, 23790-807. PMID: 22605381
4. Uyama, T. et al. (2012) The Journal of biological chemistry 287, 31905-19. PMID: 22825852
5. Mardian, EB. et al. (2015) Journal of biomedical science 22, 99. PMID: 26503625
6. Pang, XY. et al. (2012) The Journal of biological chemistry 287, 35260-35274. PMID: 22923616
