

| 货号 | HD084012 |
|---|---|
| 品牌 | abinScience |
| 描述 |
Recombinant Human NTHL1 Protein, N-His (HD084012) expressed in E. coli, spanning Gln90-Leu312. Purity: >90% by SDS-PAGE.
Highlights
|
| 表达系统 | E. coli |
| Accession号 | P78549 |
| 蛋白长度 | Gln90-Leu312 |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 种属 | Homo sapiens (Human) |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 预测分子量 | 27.01 kDa |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 别名 | Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase, DNA glycosylase/AP lyase, EC:3.2.2.-, EC:4.2.99.18, Endonuclease III-like protein 1, NTH1, NTHL1, OCTS3, hNTH1 |
| 背景 | Endonuclease III-like protein 1 is a ~33 kDa protein. Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Also has 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. 1. Williams, SC. et al. (2018) Molecular and cellular biology 38. PMID: 29610152 2. Bessho, T. (1999) Nucleic acids research 27, 979-83. PMID: 9927729 |
| Note | For research use only |

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