ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
55.20 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Angiotensin II/vasopressin receptor, NACHT, LRR and PYD domains-containing protein 6, NALP6, NLRP6, PYPAF5, PYRIN-containing APAF1-like protein 5
背景
NACHT, LRR and PYD domains-containing protein 6 is a ~98 kDa protein. Acts as the sensor component of the NLRP6 inflammasome, which mediates inflammasome activation in response to various pathogen-associated signals, leading to maturation and secretion of IL1B and IL18. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. Acts as a recognition receptor (PRR): recognizes and binds specific pathogens and other damage-associated signals, such as lipoteichoic acid (LTA), a cell-wall component of Gram-positive bacteria, or double stranded RNA (dsRNA). May also recognize and bind lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria; however, LPS is probably not a major activator of the NLRP6 inflammasome. Following LTA- or dsRNA-binding, NLRP6 undergoes liquid-liquid phase separation (LLPS), enhancing multivalent interactions, an essential step for the formation of the NLRP6 inflammasome polymeric complex.
1. Hara, H. et al. (2018) Cell 175, 1651-1664.e14. PMID: 30392956
2. Shen, C. et al. (2021) Cell 184, 5759-5774.e20. PMID: 34678144
3. Shen, C. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 2052-2057. PMID: 30674671
4. Tian, XX. et al. (2021) International endodontic journal 54, 916-925. PMID: 33377178
5. Leng, F. et al. (2020) Scientific reports 10, 198. PMID: 31932628
6. Grenier, JM. et al. (2002) FEBS letters 530, 73-8. PMID: 12387869
7. Xing, J. et al. (2021) Cell reports 35, 109205. PMID: 34161762
