ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
49.44 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Monarch-1, NACHT, LRR and PYD domains-containing protein 12, NALP12, NLRP12, PYPAF7, PYRIN-containing APAF1-like protein 7, RNO, Regulated by nitric oxide
背景
NACHT, LRR and PYD domains-containing protein 12 is a ~120 kDa protein. Plays an essential role as an potent mitigator of inflammation. Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways. Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway. In turn, promotes bacterial tolerance. Also inhibits the RIGI-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked RIGI degradation.
1. Normand, S. et al. (2018) Nature communications 9, 5338. PMID: 30559449
2. Gerhard, DS. et al. (2004) Genome research 14, 2121-7. PMID: 15489334
3. Arthur, JC. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 6291-6. PMID: 17947705
4. Chen, ST. et al. (2019) Cell host & microbe 25, 602-616.e7. PMID: 30902577
