ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
27.85 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
APE, Akt phosphorylation enhancer, CCDC88A, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, GRDN, Girders of actin filament, Girdin, HkRP1, Hook-related protein 1, KIAA1212
背景
Girdin is a ~216 kDa protein. Bifunctional modulator of guanine nucleotide-binding proteins (G proteins). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits. Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS. Essential for cell migration. Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration.
1. Garcia-Marcos, M. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 3178-83. PMID: 19211784
2. Gupta, V. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, E5721-30. PMID: 27621449
3. Lin, C. et al. (2011) Science signaling 4, ra64. PMID: 21954290
4. López-Sánchez, I. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 5510-5. PMID: 23509302
5. Lin, C. et al. (2014) Molecular biology of the cell 25, 3654-71. PMID: 25187647
6. Enomoto, A. et al. (2005) Developmental cell 9, 389-402. PMID: 16139227
7. Ghosh, P. et al. (2010) Molecular biology of the cell 21, 2338-54. PMID: 20462955
