

| 货号 | HD331012 |
|---|---|
| 品牌 | abinScience |
| 描述 |
Recombinant Human USP53 Protein, N-His (HD331012) expressed in E. coli, spanning Ala27-Cys380. Purity: >90% by SDS-PAGE.
Highlights
|
| 表达系统 | E. coli |
| Accession号 | Q70EK8 |
| 蛋白长度 | Ala27-Cys380 |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 种属 | Homo sapiens (Human) |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 预测分子量 | 42.73 kDa |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 别名 | EC:3.4.19.12, KIAA1350, USP53, Ubiquitin carboxyl-terminal hydrolase 53, Ubiquitin-specific peptidase 53 |
| 背景 | Ubiquitin carboxyl-terminal hydrolase 53 is a ~120 kDa protein. Deubiquitinase that mediates 'Lys-63'-linked deubiquitination of tight junction proteins, such as MARVELD2 and LSR, and which is involved in the survival of auditory hair cells and hearing. Specifically cleaves 'Lys-63'-linked polyubiquitin chains composed of at least 3 ubiquitin molecules, while it is not able to deubiquitinate substrates with shorter ubiquitin chains: recognizes ubiquitin chain in position S2 and catalyzes en bloc cleavage of polyubiquitin chains from substrate proteins. Probably acts by modulating the barrier properties and mechanical stability of tight junctions via deubiquitination of MARVELD2 and LSR. 1. Zhang, J. et al. (2020) Liver international : official journal of the International Association for the Study of the Liver 40, 1142-1150. PMID: 32124521 2. Wendrich, K. et al. (2025) Nature chemical biology 21, 746-757. PMID: 39587316 |
| Note | For research use only |

SDS-PAGE for Recombinant Human USP53 Protein, N-His



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