ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
30.58 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
E3 ubiquitin-protein ligase SIAH1 is a ~31 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation.
1. Nagano, Y. et al. (2003) The Journal of biological chemistry 278, 51504-14. PMID: 14506261
2. Fanelli, M. et al. (2004) The Journal of biological chemistry 279, 5374-9. PMID: 14645235
3. Germani, A. et al. (2003) Oncogene 22, 8845-51. PMID: 14654780
4. Liani, E. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 5500-5. PMID: 15064394
5. Santelli, E. et al. (2005) The Journal of biological chemistry 280, 34278-87. PMID: 16085652
6. Szargel, R. et al. (2009) The Journal of biological chemistry 284, 11706-16. PMID: 19224863
7. Buchwald, M. et al. (2010) Leukemia 24, 1412-21. PMID: 20508617
8. Liu, M. et al. (2012) Molecular cell 46, 325-34. PMID: 22483617
9. Ji, L. et al. (2017) Genes & development 31, 904-915. PMID: 28546513
10. Buratti, J. et al. (2021) Journal of medical genetics 58, 205-212. PMID: 32430360
Note
For research use only
图片
SDS-PAGE
SDS-PAGE for Human SIAH1 Recombinant Protein (N-His)
