ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
23.51 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
ARHGAP34, FNBP2, Formin-binding protein 2, KIAA0456, Rho GTPase-activating protein 34, SLIT-ROBO Rho GTPase-activating protein 2, SRGAP2, SRGAP2A, srGAP2
背景
SLIT-ROBO Rho GTPase-activating protein 2 is a ~120 kDa protein. Postsynaptic RAC1 GTPase activating protein (GAP) that plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex. Regulates excitatory and inhibitory synapse maturation and density in cortical pyramidal neurons. SRGAP2/SRGAP2A limits excitatory and inhibitory synapse density through its RAC1-specific GTPase activating activity, while it promotes maturation of both excitatory and inhibitory synapses through its ability to bind to the postsynaptic scaffolding protein HOMER1 at excitatory synapses, and the postsynaptic protein GPHN at inhibitory synapses. Mechanistically, acts by binding and deforming membranes, thereby regulating actin dynamics to regulate cell migration and differentiation. Promotes cell repulsion and contact inhibition of locomotion: localizes to protrusions with curved edges and controls the duration of RAC1 activity in contact protrusions.
1. Guo, S. et al. (2010) The Journal of biological chemistry 285, 35133-41. PMID: 20810653
2. Fossati, M. et al. (2016) Neuron 91, 356-69. PMID: 27373832
3. Sporny, M. et al. (2017) Molecular biology and evolution 34, 1463-1478. PMID: 28333212
4. Charrier, C. et al. (2012) Cell 149, 923-35. PMID: 22559944
5. Mason, FM. et al. (2011) The Journal of biological chemistry 286, 6577-86. PMID: 21148482
