ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
37.58 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Diacylglycerol O-acyltransferase 1 is a ~56 kDa protein. Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly.
1. Yen, CL. et al. (2005) Journal of lipid research 46, 1502-11. PMID: 15834126
2. Shih, MY. et al. (2009) The Journal of biological chemistry 284, 4292-9. PMID: 19028692
3. McFie, PJ. et al. (2010) The Journal of biological chemistry 285, 37377-87. PMID: 20876538
4. Qi, J. et al. (2012) Journal of lipid research 53, 1106-16. PMID: 22493088
5. Ma, Z. et al. (2017) Journal of lipid research 58, 1091-1099. PMID: 28420705
6. Buhman, KK. et al. (2002) The Journal of biological chemistry 277, 25474-9. PMID: 11959864
