ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
18.37 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
CSDD1, LIN28, LIN28A, Lin-28A, Protein lin-28 homolog A, ZCCHC1, Zinc finger CCHC domain-containing protein 1
背景
Protein lin-28 homolog A is a ~22 kDa protein. RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA.
1. Jin, J. et al. (2011) Nucleic acids research 39, 3724-34. PMID: 21247876
2. Heo, I. et al. (2008) Molecular cell 32, 276-84. PMID: 18951094
3. Heo, I. et al. (2009) Cell 138, 696-708. PMID: 19703396
4. Piskounova, E. et al. (2011) Cell 147, 1066-79. PMID: 22118463
5. Thornton, JE. et al. (2012) RNA (New York, N.Y.) 18, 1875-85. PMID: 22898984
