ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
33.29 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
HsRAD51AP1, PIR51, RAD51-associated protein 1, RAD51-interacting protein, RAD51AP1
背景
RAD51-associated protein 1 is a ~38 kDa protein. Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2.
1. Modesti, M. et al. (2007) Molecular cell 28, 468-81. PMID: 17996710
2. Wiese, C. et al. (2007) Molecular cell 28, 482-90. PMID: 17996711
3. Dray, E. et al. (2010) Nature structural & molecular biology 17, 1255-9. PMID: 20871616
4. Parplys, AC. et al. (2014) DNA repair 24, 87-97. PMID: 25288561
5. Parplys, AC. et al. (2015) Nucleic acids research 43, 9817-34. PMID: 26323318
6. Dunlop, MH. et al. (2012) The Journal of biological chemistry 287, 12343-7. PMID: 22375013
