ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
20.85 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
EC:3.1.-.-, Endoribonuclease ZC3H12A, MCP-induced protein 1, MCPIP, MCPIP-1, MCPIP1, Monocyte chemotactic protein-induced protein 1, Reg1, Regnase-1, ZC3H12A, Zinc finger CCCH domain-containing protein 12A
背景
Endoribonuclease ZC3H12A is a ~65 kDa protein. Endoribonuclease involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay. Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation. Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines (IL6 and IL2), cell surface receptors (ICOS, TNFRSF4 and TNFR2) and transcription factor (REL). Inhibits cooperatively with ZC3H12A the differentiation of helper T cells Th17 in lungs.
1. Mino, T. et al. (2015) Cell 161, 1058-1073. PMID: 26000482
2. Matsushita, K. et al. (2009) Nature 458, 1185-90. PMID: 19322177
3. Liang, J. et al. (2010) The Journal of experimental medicine 207, 2959-73. PMID: 21115689
4. Li, M. et al. (2012) PloS one 7, e49841. PMID: 23185455
5. Uehata, T. et al. (2013) Cell 153, 1036-49. PMID: 23706741
10. Huang, S. et al. (2015) The Journal of biological chemistry 290, 20782-20792. PMID: 26134560
